性别: 男
职称:副教授
导师类型: 硕士生导师
学科方向: 071004-水生生物学
研究方向：1.水生甲壳动物抗病、抗逆、生长的协同拮抗机制
2.凡纳滨对虾抗病、抗逆、生长优良性状的分子设计育种
个人经历
本硕博就读于600全讯白菜官方网站，2023年2月成为副教授，隶属于水产动物疫病防控健康养殖全国重点实验室主任何建国教授团队。
目前研究
目前多数养殖品种的选育手段依然停留于针对单一性状、依靠群体表型选育的传统方式，选育周期长，群体保育和养殖工作量大。而针对生长、抗病等多性状的综合改良品种选育则缺乏投入和研究。由于国内养殖区域的多样化、养殖环境的变化以及养殖模式的转变，凡纳滨对虾养殖迫切需要更多具有综合优势的新品种。目前分子设计育种技术成熟，然而凡纳滨对虾生长、抗病、抗逆的功能基因的研究不足，已成为限制培育优良新品种的重要因素。针对这些产业问题和科学问题，本项目组目前有以下研究方向：
（1）凡纳滨对虾氨氮排放和抗病毒通路的拮抗机制研究。本研究方向主要聚焦干扰素途径和Wnt/β-catenin途径的拮抗机制，挖掘具有育种价值的解耦拮抗机制的靶标基因。
（2）凡纳滨对虾抗亚硝酸胁迫和快生长的协同机制研究。亚硝酸盐在养殖过程不可避免的累积已经成为了威胁养殖行业的环境胁迫之一，生长一直是凡纳滨对虾育种的最关键性状。本方向主要解析凡纳滨对虾抗亚硝酸胁迫和快生长协同机制的分子机制，挖掘具有育种价值的解耦拮抗机制的靶标基因。2025年需要招收工程硕士1名（生物与医药）。
（3）凡纳滨对虾抗病毒和快生长的协同机制研究。本研究方向主要解析凡纳滨对虾抗WSSV和DIV1的分子机制，阐明抗病毒途径和生长通路的协同机制，挖掘具有育种价值的抗病生长的靶标基因。2025年需要招收学术硕士1名（水生生物学）。
（4）水生甲壳动物先天免疫的遗传演化机制。本研究方向主要围绕凡纳滨对虾解析水生甲壳动物先天免疫机制，阐明其遗传演化机制，挖掘具有育种价值的先天免疫靶标基因。2025年需要招收工程硕士1名（生物与医药）。
欢迎有兴趣的同学投递简历到 lihy88@mail.sysu.edu.cn。
学术成就
一、水生甲壳动物先天免疫机制研究
对虾是我国重要的水产动物养殖种类，2021年我国对虾养殖产量超过227万吨，位居世界第一。我国对虾养殖业经历过两次重大危机，均由病害导致。第一次危机是由白斑综合征病毒（WSSV）引发，曾导致我国养殖对虾损失70%。第二次危机是弧菌等多种条件致病菌引发的细菌性疾病，曾导致全球损失23%，我国损失17%。本项目组针对对虾病毒和细菌性疾病开展抗病毒和抗细菌先天性免疫研究，取得了具有原创性的研究成果。
（1）揭示了对虾Toll介导的抗WSSV免疫新通路，挖掘了对虾p53调控Toll通路的新机制。干扰素通路是对虾中发现的第一条抗病毒通路，本项目组挖掘出第二条对虾抗病毒（WSSV）途径。Toll是进化保守的模式识别受体，研究发现了WSSV特异性识别受体Toll4，阐明了Toll4通过Dorsal诱导抗脂多糖因子（ALF）家族和溶菌酶（LYZ）家族蛋白表达的调控机制，揭示了ALF和LYZ通过结合病毒囊膜蛋白发挥清除病毒功能。研究成果在病原学顶级期刊PLoS Pathogens杂志上发表。抑癌基因p53参与肿瘤形成、免疫应答等生命过程。项目组阐明了对虾p53通过与Dorsal互作调控Toll通路的新机制，首次证明无脊椎动物p53参与先天免疫。Cell综述文章“Putting p53 in Context”评述该成果，指出p53在进化上参与先天免疫调控要早于抑制肿瘤发生。
（2）发现了对虾STING介导的抗弧菌免疫新途径。病毒和细菌感染宿主过程中暴露出双链DNA能够激活先天免疫。Toll家族TLR9最早被鉴定为DNA识别受体，但不是DNA识别应答的必要条件。最新研究表明cGAS-STING通路识别病原性双链DNA启动免疫应答，是DNA激活免疫的主要途径。项目组发现了对虾STING介导的抗弧菌免疫新途径，阐明了STING-IKKβ-Relish诱导抗菌肽表达的调控机制，率先证明无脊椎动物STING参与抗细菌免疫。Cell子刊Trends in Immunology综述文章“Evolutionary Origins of cGAS-STING Signaling”评述该成果，指出STING介导NF-κB途径的保守性及可能的功能演化历程将成为未来研究的重点。
二、水生甲壳动物环境适应性机制研究
（1）揭示氨氮胁迫诱发副溶血弧菌爆发的分子机制。水体氨氮胁迫是凡纳滨对虾养殖过程的环境胁迫之一，本项目组发现氨氮胁迫阻断凡纳滨对虾白介素I受体同源基因（interleukin 1 receptor, IL-1R）介导的抗细菌途径，首次揭示水体氨氮胁迫降低对虾抵抗弧菌感染的分子机制。
（2）阐明红螯螯虾适应低氧环境的分子遗传机制。本项目组构建了红螯螯虾染色体及基因组，通过比较基因组学分析发现低氧胁迫相关基因KDM5A受到正选择影响，并利用敲低实验证明KDM5A能够提高红螯螯虾耐低氧能力，为红螯螯虾低氧耐受性提供了基因组基础。
（3）挖掘高温驱动的拟穴青蟹卵巢发育异常的分子机制。黄油蟹，因为卵巢发育异常，卵黄蛋白外溢，形成特别的风味，而被誉为“蟹中之王”，是粤港澳地区新兴名贵蟹种，以环境和天气影响产量质量，生产期长，价格高昂，每50克数百港元，在当地极受欢迎。本项目组通过联系拟穴青蟹不同基因型和不同性状进行分析，解析拟穴青蟹在高温环境下的适应性机制，阐述黄油蟹产生的分子机制。该工作目前投稿《nature communications》，处于修回审稿阶段。
三、凡纳滨对虾抗细菌优良性状的分子设计育种
与传统家系选育比较，分子设计育种能够实现从“经验育种”到定向高效的“精确育种”转变，不仅能减少选育的盲目性，而且可以大幅提高目标优良性状的育种效率。本项目组已创建了针对干扰素调节因子IRF的分子设计育种技术并进行了育种实践，创制了抗细菌凡纳滨对虾“中兴2号”国审新品种。
承担课题
国家自然科学基金面上项目，凡纳滨对虾干扰素调节因子（LvIRF）调控鳃排氨的分子机制，32173000，58万，国家级，2022.1-2025.12，主持。
国家自然科学基金青年基金，凡纳滨对虾TRAF3激活IRF机制及抗病毒效应，31802326，25万，国家级，2019.1-2021.12，主持。
中国博士后科学基金面上资助，凡纳滨对虾STING介导IRF激活的抗病毒分子机制，2017M622850，5万，省部级，2018.1-2019.6，主持。
广州市科技计划项目基础与应用基础研究项目（博士青年科技人员类），凡纳滨对虾IL-1R介导抗细菌途径的激活机制的研究及应用，202102020354，5万，市级，2021.04-2023.03，主持。
高校基本科研业务费，WSSV感染凡纳滨对虾拮抗TRAF3抗病毒途径的分子机制，20lgpy55，8.31万，校级，2020.1-2021.12，主持。
论文专著
Genome assembly of redclaw crayfish (Cherax quadricarinatus) provides insights into its immune adaptation and hypoxia tolerance. Z Liu#, J Zheng#, H Li#, K Fang, S Wang, J He, D Zhou, S Weng, M Chi, Z Gu, ... BMC genomics 25 (1), 746, 2024.
Nucleic Acid Sensing by STING Induces an IFN-like Antiviral Response in a Marine Invertebrate. H Li, X Di, S Wang, Q Li, S Weng, J He, C Li. The Journal of Immunology, 2024.
Ammonia nitrogen stress increases susceptibility to bacterial infection via blocking IL-1R–Relish axis mediated antimicrobial peptides expression in shrimp. H Li, Q Li, S Wang, J He, C Li. Aquaculture 563, 738934, 2023.
The MIP-T3 from shrimp Litopenaeus vannamei restricts white spot syndrome virus infection via regulating NF-κB activation. H Li, Q Li, S Wang, J He, C Li, Fish & Shellfish Immunology 127, 56-64, 2022.
Stimulator of interferon genes (STING) defends against bacterial infection via IKKβ-mediated Relish activation in shrimp. H Li, Q Li, S Wang, J He, C Li. Frontiers in Immunology, 4678, 2022.
TNF-Receptor-Associated Factor 3 in Litopenaeus vannamei Restricts White Spot Syndrome Virus Infection Through the IRF-Vago Antiviral Pathway. H Li, Q Fu, S Wang, R Chen, X Jiang, P Zhu, J He, C Li. Frontiers in Immunology 11, 2110, 2020.
RNAi screening identifies a new Toll from shrimp Litopenaeus vannamei that restricts WSSV infection through activating Dorsal to induce antimicrobial peptides. H Li, B Yin, S Wang, Q Fu, B Xiao, K Lǚ, J He, C Li. PLoS pathogens 14 (9), e1007109, 2018.
An invertebrate STING from shrimp activates an innate immune defense against bacterial infection. H Li, S Wang, K Lǚ, B Yin, B Xiao, S Li, J He, C Li. FEBS letters 591 (7), 1010-1017, 2017.
Identification of two p53 isoforms from Litopenaeus vannamei and their interaction with NF-κB to induce distinct immune response. H Li, S Wang, Y Chen, K Lǚ, B Yin, S Li, J He, C Li. Scientific reports 7 (1), 45821, 2017.
MKK6 from pacific white shrimp Litopenaeus vannamei is responsive to bacterial and WSSV infection. H Li, S Wang, Z Qian, Z Wu, K Lǚ, S Weng, J He, C Li. Molecular immunology 70, 72-83, 2016.
A C-type lectin (LvCTL4) from Litopenaeus vannamei is a downstream molecule of the NF-κB signaling pathway and participates in antibacterial immune response. H Li, Y Chen, M Li, S Wang, H Zuo, X Xu, S Weng, J He, C Li. Fish & shellfish immunology 43 (1), 257-263, 2015.
Intestine bacterial community affects the growth of the Pacific white shrimp (Litopenaeus vannamei) . D Hou, B Yin, S Wang, H Li, S Weng, X Jiang, H Li, C Li, J He, Z Huang. Applied Microbiology and Biotechnology 108 (1), 59 2024.
Nitrite nitrogen stress disrupts the intestine bacterial community by altering host-community interactions in shrimp. D Hou, H Li, S Wang, S Weng, J He. Science of The Total Environment 925, 171536,2024.
Signaling events induced by lipopolysaccharide-activated Toll in response to bacterial infection in shrimp. S Wang, H Li, Q Li, B Yin, S Li, J He, C Li. Frontiers in Immunology 14, 1119879,2023.
An AhR–Caspase Axis Mediated Antiviral Apoptosis in an Arthropod. Q Fu, H Li, S Wang, X Liao, S Chen, B Xiao, R Chen, X Jiang, J He, C Li, The Journal of Immunology 209 (10), 2022.
Rap2a is negatively regulated by NF-κB and contributes to growth via wnt pathway in shrimp. S Wang, M He, Q Li, H Li, B Yin, J He, C Li, Frontiers in Marine Science 9, 942009, 2022.
Interferon-Induced Protein 6-16 (IFI6-16) from Litopenaeus vannamei Regulate Antiviral Immunity via Apoptosis-Related Genes. K Lǚ, H Li, S Wang, A Li, S Weng, J He, C Li, Viruses 14 (5), 1062, 2022.
TAK1 confers antibacterial protection through mediating the activation of MAPK and NF-κB pathways in shrimp. S Wang, H Li, R Chen, X Jiang, J He, C Li. Fish & shellfish immunology 123, 248-256, 2022.
Phenylalanine hydroxylase (PAH) plays a positive role during WSSV and Vibrio parahaemolyticus infection in Litopenaeus vannamei. Y Yao, L Shi, W Xiao, S Guo, S Liu, H Li, S Zhang. Fish & Shellfish Immunology 120, 515-525, 2022.
MAPKKK15 gene from shrimp litopenaeus vannamei is transcribed in larva development stages and contributes to WSSV pathogenesis. S Wang, H Li, P Zhu, Q Fu, B Yin, Q Li, R Chen, X Jiang, S Weng, J He, ...Aquaculture 534, 736324, 2021.
White spot syndrome virus establishes a novel IE1/JNK/c-Jun positive feedback loop to drive replication. S Wang, H Li, S Weng, C Li, J He, Iscience 23 (1), 2020.
Molecular cloning, expression, promoter analysis and functional characterization of a new Crustin from Litopenaeus vannamei. M Li, C Ma, H Li, J Peng, D Zeng, X Chen, C Li. Fish & shellfish immunology 73, 42-49, 2018
MKK4 from Litopenaeus vannamei is a regulator of p38 MAPK kinase and involved in anti-bacterial response. S Wang, B Yin, H Li, B Xiao, K Lǚ, C Feng, J He, C Li, Developmental & Comparative Immunology 78, 61-70, 2018.
Shrimp TAB1 interacts with TAK1 and p38 and activates the host innate immune response to bacterial infection. S Wang, M Li, B Yin, H Li, B Xiao, K Lǚ, Z Huang, S Li, J He, C Li. Molecular Immunology 88, 10-19, 2017.
Identification and functional analysis of a TEP gene from a crustacean reveals its transcriptional regulation mediated by NF-κB and JNK pathways and its broad protective roles …. C Li, H Li, B Xiao, Y Chen, S Wang, K Lǚ, B Yin, S Li, J He. Developmental & Comparative Immunology 70, 45-58, 2017.
Shrimp with knockdown of LvSOCS2, a negative feedback loop regulator of JAK/STAT pathway in Litopenaeus vannamei, exhibit enhanced resistance against WSSV. S Wang, X Song, Z Zhang, H Li, K Lǚ, B Yin, J He, C Li, Developmental & Comparative Immunology 65, 289-298,2016.
Cloning, identification and functional analysis of a β-catenin homologue from Pacific white shrimp, Litopenaeus vannamei. S Zhang, L Shi, K Lǚ, H Li, S Wang, J He, C Li, Fish & shellfish immunology 54, 411-418,2016.
Identification and characterization of transforming growth factor β-activated kinase 1 from Litopenaeus vannamei involved in anti-bacterial host defense. S Wang, H Li, K Lǚ, Z Qian, S Weng, J He, C Li, Fish & shellfish immunology 52, 278-288, 2016.
Identification and characterization of MKK7 as an upstream activator of JNK in Litopenaeus vannamei. S Wang, Z Qian, H Li, K Lǚ, X Xu, S Weng, J He, C Li, Fish & Shellfish Immunology 48, 285-294, 2016.
Activation of Vago by interferon regulatory factor (IRF) suggests an interferon system-like antiviral mechanism in shrimp. C Li, H Li, Y Chen, Y Chen, S Wang, SP Weng, X Xu, J He. Scientific reports 5 (1), 15078, 2015.
Identification and functional characterization of the TAB2 gene from Litopenaeus vannamei. S Wang, H Li, Z Qian, X Song, Z Zhang, H Zuo, X Xu, S Weng, J He, C Li. Fish & Shellfish Immunology 46 (2), 206-216, 2015.
The c-Fos and c-Jun from Litopenaeus vannamei play opposite roles in Vibrio parahaemolyticus and white spot syndrome virus infection. C Li, H Li, S Wang, X Song, Z Zhang, Z Qian, H Zuo, X Xu, S Weng, J He. Developmental & Comparative Immunology 52 (1), 26-36, 2015.
Daxx from Pacific white shrimp Litopenaeus vannamei is involved in activation of NF-κB pathway. M Yan, J Tang, Q Liang, G Zhu, H Li, C Li, S Weng, J He, X Xu, Fish & shellfish immunology 45 (2), 443-453, 2015.
A Janus Kinase in the JAK/STAT signaling pathway from Litopenaeus vannamei is involved in antiviral immune response. X Song, Z Zhang, S Wang, H Li, H Zuo, X Xu, S Weng, J He, C Li. Fish & Shellfish Immunology 44 (2), 662-673, 2015.
Identification of a JAK/STAT pathway receptor domeless from Pacific white shrimp Litopenaeus vannamei. M Yan, C Li, Z Su, Q Liang, H Li, S Liang, S Weng, J He, X Xu. Fish & Shellfish Immunology 44 (1), 26-32, 2015.
Identification and functional analysis of a Hemolin like protein from Litopenaeus vannamei. H Zuo, H Li, E Wei, Z Su, J Zheng, C Li, Y Chen, S Weng, J He, X Xu. Fish & Shellfish Immunology 43 (1), 51-59, 2015.
Identification of a C-type lectin with antiviral and antibacterial activity from pacific white shrimp Litopenaeus vannamei. M Li, C Li, C Ma, H Li, H Zuo, S Weng, X Chen, D Zeng, J He, X Xu. Developmental & Comparative Immunology 46 (2), 231-240, 2014.
Pellino protein from pacific white shrimp Litopenaeus vannamei positively regulates NF-κB activation. C Li, J Chai, H Li, H Zuo, S Wang, W Qiu, S Weng, J He, X Xu. Developmental & Comparative Immunology 44 (2), 341-350, 2014.
Presence of Tube isoforms in Litopenaeus vannamei suggests various regulatory patterns of signal transduction in invertebrate NF-κB pathway. C Li, Y Chen, S Weng, S Li, H Zuo, X Yu, H Li, J He, X Xu. Developmental & Comparative Immunology 42 (2), 174-185, 2014.
