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学术预告
Transcriptome and Proteome Changes in Response to Perturbation of pre-mRNA Splicing during Reproduction in Arabidopsis
活动日期：
2018-05-21
活动时间：
10:00-11:30
主讲嘉宾：
张晓宁 教授
主持人：
李剑峰 教授
地点：
600全讯白菜官方网站生物楼205会议室
pre-mRNA splicing is a conserved process in all eukaryotic cells. Regulation of splicing can lead to diverse mRNA and protein products and affect related biological processes. To understand how splicing regulation plays a role in plant reproduction, we investigated transcriptome and proteome responses when splicing is misregulated in inflorescence using an established splicing regulator null mutant, sr45-1. Serine/Arginine-rich 45 (SR45) has recently been shown to be a neutral regulator in the splicing machinery. It regulates pre-mRNA via interactions with other regulatory proteins and spliceosome subunits. The sr45-1 null mutant exhibits pleiotropic phenotypes, including narrower flower petal and reduced seed yield, suggesting that SR45 regulates genes involved in diverse bioprocesses in reproduction. We performed RNA-seq and RIP-seq analysis with RNAs from inflorescence. Using different bioinformatics tools, we were able to extract SR45-differentially regulated (SDR) genes, SR45-dependent alternative splicing (SAS) events and SR45-associated RNAs (SARs), separately. Reproductive process genes are significantly overrepresented in SARs, but not in SDR genes and SR45-dependent AS genes. We also compared the inflorescence proteome between wild-type and sr45-1 using TMT6 quantitative protein profiling. Ongoing Meta-analysis results between transcriptome and proteome studies will help understand how SR45-regulated mRNAprotein network contributes to plant reproduction.
